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Depending on the application the IONUS PCD is equipped with one Photometer. Further equipment can be requested on demand. 440 nm: Bromate according to EPA 317 or organic acids; 355 nm: Bromate according to EPA326, 530 nm: Transition metals.
High pressure side prime and purge valves in eluent and reagent flow.
Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).
The IONUS can be equipped with an additional UV/VIS detector.
| Technical Specifications | |
|---|---|
| method | ion exchange chromatography with post column derivatization |
| manual injection | 20 µL, further injection loops upon request (50 µL, 100 µL, 200 µL) |
| autosampler | 2 racks, each of them with space for 48 vials (1.5 mL) or 12 vials (6 mL), sample cooling, loop 100 µL, 3 injection modes (full-loop, partial loop, microliter-pickup) |
| column | stainless steel / PEEK |
| temperature stability / range | ~ 0.5 °C / ambient up to 50 °C |
| detection | post column derivatization (PCD) with a singe wavelength photometer in UV-VIS range (440 nm, 355 nm, 530 nm) depending on application |
| isocratic pump system | max. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min reproducibility of flow rate: 0.1 % RSD at 100 µL/min |
| maximum operating pressure | 120 bar |
| control software & data aquisition | Clarity (21 CFR part 11 compliant) or AminoPeak |
| dimensions, weight, power | 1000 x 350 x 375 mm*, 60 kg, 110 - 230 V, 50/60 Hz |
| *800 mm with opened main unit |
Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization and photometric determination by LED. Reagents that can be used are dianisidine and triiodide for the study of bromate, pyridylazoresorcinol (PAR) for transition metals and bromothymol blue for organic acids.
The samples for the IONUS PCD are mainly water-based. Analyses according to EPA 317 and EPA 326 are standard.
The autsoampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.
The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.
The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.
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