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Ionus PCD Ion Chromatograph

Using post-column derivatization

OVERVIEW

Designed for very low detection levels and complex detection in Ion Chromatography

  • This generally more complex detection technique is used if the detection by means of conductivity provides only very small peaks, so that the sensitivity is quite low or this optical detection is much more sensitive.
  • This can reduce the detection limit by orders of magnitude, as for example with bromate to less than 1 ppb.
  • This IONUS Post Column Derivatization (PCD) does not have a built-in conductivity detector. Instead, it has a reactor for derivatization followed by a single wavelength photometer.
  • Different wavelengths are required for these special applications and used colour reactions. These single wavelength photometers are very sensitive compared to a variable UV/VIS detector, and it can be easily changed by the user.
  • The choice of wavelength depends on the selected colour reaction, such as for the two bromate determinations according to EPA 317 or 326 with 440 nm or 355 nm.
  • The IONUS PCD is able to detect Chromium (III) / Chromium VI) with 340 nm or 530 nm
  • This IONUS PCD also offers the advantage that it can be used with IC columns of all manufacturers and is compatible with all common eluents of anion and cation chromatography.
IONUS Ion Chromatograph Product View

Main technical specifications of the system​

Flowchart

  • This IONUS Post Column Derivatization (PCD) does not have a built-in conductivity detector. Instead, it has a reactor for derivatization followed by a single wavelength photometer.
  • Different wavelengths are required for these special applications and used colour reactions. These single wavelength photometers are very sensitive compared to a variable UV/VIS detector, and it can be easily changed by the user.
  • The choice of wavelength depends on the selected colour reaction, such as for the two bromate determinations according to EPA 317 or 326 with 440 nm or 355 nm.
FlowcharT: IONUS PCD
Options: Photometer Ionus PCD

Photometer

Depending on the application the IONUS PCD is equipped with one Photometer. Further equipment can be requested on demand. 440 nm: Bromate according to EPA 317 or organic acids; 355 nm: Bromate according to EPA326, 530 nm: Transition metals.

Options: Purge valve IONUS

Prime and purge valves

High pressure side prime and purge valves in eluent and reagent flow.

Options: Software Aracus

Software 21 CFR Part 11 compliance

Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).

Options: Detector Ionus

Indirect UV detection

The IONUS can be equipped with an additional UV/VIS detector.

Technical Specifications
methodion exchange chromatography with post column derivatization
manual injection20 µL, further injection loops upon request (50 µL, 100 µL, 200 µL)
autosampler2 racks, each of them with space for 48 vials (1.5 mL) or 12 vials (6 mL), sample cooling, loop 100 µL, 3 injection modes (full-loop, partial loop, microliter-pickup)
columnstainless steel / PEEK
temperature stability / range~ 0.5 °C / ambient up to 50 °C
detectionpost column derivatization (PCD) with a singe wavelength
photometer in UV-VIS range (440 nm, 355 nm, 530 nm)
depending on application
isocratic pump systemmax. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min
reproducibility of flow rate: 0.1 % RSD at 100 µL/min
maximum operating pressure120 bar
control software & data aquisitionClarity (21 CFR part 11 compliant) or AminoPeak
dimensions, weight, power1000 x 350 x 375 mm*, 60 kg, 110 - 230 V, 50/60 Hz
*800 mm with opened main unit

FAQ

Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization and photometric determination by LED. Reagents that can be used are dianisidine and triiodide for the study of bromate, pyridylazoresorcinol (PAR) for transition metals and bromothymol blue for organic acids.

The samples for the IONUS PCD are mainly water-based. Analyses according to EPA 317 and EPA 326 are standard.

The autsoampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.

The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.

The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.

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Analysis of the amino acid composition of cell culture media
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