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The chart shows the structure of the classic setup:
Upon request each system can be supplied as manual configuration.
High pressure side prime and purge valves in eluent and reagent flow.
Validation documents IQ/OQ and PQ for ARACUS.
Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).
It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.
Active control of fluid volume by weight cell of each bottle. (only available with software aminoPeak).
IVD kit for the qualitative and quantitative determination of amino acids in physiological fluids like human blood and urine for medical diagnostics in combination with the ARACUS IVD classic.
| Technical Specifications | |
|---|---|
| method | post column derivatization with Ninhydrin |
| autosampler | 2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 - 50 μL), microliter pickup (1 - 35 μL) |
| column | stainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD |
| column oven | 20 - 100 °C, accuracy 0.1 % |
| reactor temperature | 20 - 140 °C |
| spectroscopic detection | measuring range: high, low LED photometers (440 nm + 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty |
| detection limit | < 40 pmol / 20 µL with Ninhydrin |
| quantification limit | > 100 pmol / 20 µL with Ninhydrin |
| pump system | single pump, reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min |
| maximum operating pressure | 120 bar |
| ready to use kits | kits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 - 1000 runs |
| columns & eluents | Compatible with all columns, operating with all eluents based on different ionic strength (Li, Na, K) |
| control software & data aquisition | Clarity (21 CFR part 11 compliant) or AminoPeak |
| dimensions, weight, power | 1080 x 370 x 375 mm*, 60 kg, 110 - 230 V |
| *800 mm with opened main unit |
Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization with ninhydrin (Stein & Moore) and photometric determination using LED.
The autosampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.
All applications dealing with amino acid analysis can be covered. These include the analysis of food and feed, the analysis of clinical samples such as blood plasma, urine or spinal fluid as well as research questions.
If the system is operated with the Clarity software, this option is possible. Contact us.
The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.
The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.
You can determine the heating profile of the column oven, the temperature of the reactor, the flow of the eluents, and the selection of the eluents yourself. Optimized programs are delivered with the system as standard.
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