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ARACUS classic Amino Acid Analyzer

Standard configuration with 2 photometer LEDs

TESTIMONIALS

Jing WangNational research Institute of Food and Fermentation Industries, China
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"Aracus has a local model (A300) in China. It has been 6 years since we first used this excellent instrument in our lab. It is very convenient for analyzing all kinds of hydrolyzed and free amino acids, easy to use as every process is fully automatic. Resolution and reproducibility are excellent compared to pharmacopoeia or official measurement documents. We mainly use the system to analyze feedstuff, food and health-care products, the results always have a high accuracy. It’s very user-friendly!"
Elena BudkevichNorth Caucasus Federal University, Russia
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"We have been using Aracus since 2014. Through this time we have analyzed different types of probes from rat saliva to new drugs with high resolution and reproducibility. During the exploitation period there was some problem with equipment. All problems have been solved by local after sale service and their German colleague."
Warren KrugerFox Chase Cancer Center, USA
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"Replaced our 20 year old BioChrom30. Uses much less reagent and has nearly identical performance. Software is easy to use. Support has been excellent. Highly recommended."
Alice JiangNational University of Singapore
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"This analyzer is easy to use in terms of sample preparation, sample run as well as data analysis. The results are reproducible. However, we did run into several problems, such as SD card fault, pump fault, unstable pressure. The service team and engineer helped resolve the problems every time. In general I'm satisfied with their product and service. It would be better if there were local service support so that we do not have to go back and forth with Germany all the time."
Maria-Luise WunschWarsteiner Brewery, Germany
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"The device has been in operation with us since 2009. It provides reproducible results. It is durable and low maintenance. Minor faults such as leaks and blockages can be remedied due to the clarity and accessibility of the device itself. Long lead times or runtimes."
Xu ZhangSichuan University, China
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"This instrument is easy to use and can obtain results without complex operation. Great precision, resolution, repeatability, stability and accuracy."
Anastasia SerkovaVolgograd state Technical University, Russia
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"Sensitivity and reproducibility are sufficient for our work. The software is easy-to-use and intuitive and maintenance is also quite easy. Problems arose after improper preservation of the instrument, but a service engineer helped fix the problem. Sufficiently long-term sample preparation of meat and dairy products."
Guifen TangHenan University of Animal Husbandry and Economy, China
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"From the point of view of the operator it is easy to use. Being an uncomplicated and user-friendly device, the equipment has sufficient precision and accuracy. Its other great virtue is durability and stable product quality. We have been using the equipment for almost five years, it is still running stably and is of great help in scientific research.".
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OVERVIEW

Amino Acid Analysis for a wide range of applications

  • The setup of the ARACUS „classic“ is the standard configuration of the analyzer series.
  • The ARACUS „classic“ allows the continous analysis of 96 samples in a cooled autosampler.
  • Eluents and reagent solution are selectively chosen through valves and are ideally mixed to each other.
  • The addition of ninhydrin provides a quantitative analysis statement
  • A washing routine of the injection valve and syringe pump avoids cross contaminations.
  • The level of the individual reagents can be monitored during the runs and warns the user if the quantity is no longer sufficient.

Main technical specifications of the system​

Flowchart

The chart shows the structure of the classic setup:

  • The pump allows the simultaneous operation of two fluidic lines (eluent side and reactive side), which guarantees a constant mixing.
  • The ARACUS “classic” has two photometers, 440 nm and 570 nm.
  • The system is equipped with an autosampler with 100 µL loop.
Flowchart: ARACUS classic
Options: Manual injection Aracus

Manual injection port

Upon request each system can be supplied as manual configuration.

Options: Purge valve Aracus

Prime and purge valves

High pressure side prime and purge valves in eluent and reagent flow.

Options: IQQ Documents Aquinity P10

IQ/OQ/PQ documents

Validation documents IQ/OQ and PQ for ARACUS.

Options: Software Aracus

Software 21 CFR Part 11 compliance

Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).

Options: Inert gas Aracus

Inert gas

It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.

Options: Levelsensor Aracus

Level control

Active control of fluid volume by weight cell of each bottle. (only available with software aminoPeak).

Options: IVD Kit Aracus

InVitro diagnostic kit IVDD 98/79 ED

IVD kit for the qualitative and quantitative determination of amino acids in physiological fluids like human blood and urine for medical diagnostics in combination with the ARACUS IVD classic.

Technical Specifications
methodpost column derivatization with Ninhydrin
autosampler2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 - 50 μL), microliter pickup (1 - 35 μL)
columnstainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD
column oven20 - 100 °C, accuracy 0.1 %
reactor temperature20 - 140 °C
spectroscopic detectionmeasuring range: high, low LED photometers (440 nm + 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty
detection limit< 40 pmol / 20 µL with Ninhydrin
quantification limit> 100 pmol / 20 µL with Ninhydrin
pump systemsingle pump, reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min
maximum operating pressure120 bar
ready to use kitskits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 - 1000 runs
columns & eluentsCompatible with all columns, operating with all eluents based on different ionic strength (Li, Na, K)
control software & data aquisitionClarity (21 CFR part 11 compliant) or AminoPeak
dimensions, weight, power1080 x 370 x 375 mm*, 60 kg, 110 - 230 V
*800 mm with opened main unit

FAQ

Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization with ninhydrin (Stein & Moore) and photometric determination using LED.

The autosampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.

All applications dealing with amino acid analysis can be covered. These include the analysis of food and feed, the analysis of clinical samples such as blood plasma, urine or spinal fluid as well as research questions.

If the system is operated with the Clarity software, this option is possible. Contact us.

The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.

The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.

You can determine the heating profile of the column oven, the temperature of the reactor, the flow of the eluents, and the selection of the eluents yourself. Optimized programs are delivered with the system as standard.

Complete your name and email to receive the application note.

Analysis of the amino acid composition of cell culture media
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