Play Video

ARACUS lite Amino Acid Analyzer

Small sample throughput and less eluent consumption


Amino Acid Analysis in a simplified configuration

  • The ARACUS „lite“ is a simplified configuration of the ARACUS „classic“ and designed for a reduced number of samples and smaller available budget.
  • Due to the small sample throughput and less eluent consumption the bottles of eluents are reduced in size and makes it possible to store the eluent bottles inside of the device and the rack .
  • For analysis of 1° amino acids with 570 nm, for 1° and 2° with 500 nm.
  • The system can be upgraded to a dual wavelength photometer with 440 and 570 nm by replacing the already installed photometer.
Amino Acid Analyzer: Aracus Lite

Main technical specifications of the system​


The chart shows the structure of the lite setup:

    • The ARACUS „lite“ is using the same pump configuration for operation of two fluidic lines (eluent side and reactive side) and suitable for High Speed and High Resolution columns.
    • The „lite“ comes just with one LED photometer depending on the application.
    • The system equipped with manual injection mode can be connect also with an autosampler like all our devices.
    Flowchart: ARACUS lite
    Options: Autosamper Aracus


    The ARAUS lite can be upgraded later on with autosampler.

    Options: Purge valve Aracus

    Prime and purge valves

    High pressure side prime and purge valves in eluent and reagent flow.

    Options: IQQ Documents Aquinity P10

    IQ/OQ/PQ documents

    Validation documents IQ/OQ and PQ for ARACUS.

    Options: Software Aracus

    Software 21 CFR Part 11 compliance

    Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).

    Options: Inert gas Aracus

    Inert gas

    It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.

    Options: Dual Photometer Aracus

    Dual wavelength photometer

    The existing 500 or 570 nm photometer will be replaced by a photometer with 440 nm and 570 nm wavelength.

    Technical Specifications
    methodpost column derivatization with Ninhydrin
    autosamplerARACUS lite can be upgraded with autosampler;
    2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 - 50 μL), microliter pickup (1 - 35 μL)
    columnstainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD
    column oven20 - 100 °C, accuracy 0.1 %
    reactor temperature20 - 140 °C
    spectroscopic detectionmeasuring range: high, low LED photometer (570 nm or 500 nm), or dual photometer (440 # 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty
    detection limit< 40 pmol / 20 µL with Ninhydrin
    quantification limit> 100 pmol / 20 µL with Ninhydrin
    pump systemsingle pump reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min
    maximum operating pressure120 bar
    ready to use kitskits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 - 1000 runs
    columns & eluentsCompatible with all High-Speed and High-Resolution columns, operating with all eluents based on different ionic strength (Li, Na, K)
    control software & data aquisitionClarity (21 CFR part 11 compliant) or AminoPeak
    dimensions, weight, power1080 x 370 x 375 mm*, 60 kg, 110 - 230 V
    *800 mm with opened main unit


    Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization with ninhydrin (Stein & Moore) and photometric determination using LED.

    Yes, this is possible as with all ARACUS systems running on manual injection.

    The autsoampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.

    All applications dealing with amino acid analysis can be covered. These include the analysis of food and feed, the analysis of clinical samples such as blood plasma, urine or spinal fluid as well as research questions.

    The ARACUS lite uses only a single photometer with a wavelength of 500 nm instead of two photometers with wavelengths of 570 and 440 nm. As an option, we offer a dual photometer that covers both wavelengths. In addition, the system has smaller bottles for the eluents, which eliminates the eluent rack and makes the system even more compact.

    The system is requested when there is a lower volume of samples.

    If the system is operated with the Clarity software, this option is possible. Contact us.

    The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.

    The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.

    You can determine the heating profile of the column oven, the temperature of the reactor, the flow of the eluents, and the selection of the eluents yourself. Optimized programs are delivered with the system as standard.

    Complete your name and email to receive the application note.

    Analysis of the amino acid composition of cell culture media
    Get download link