The chart shows the structure of the lite setup:
|method||post column derivatization with Ninhydrin|
|autosampler||ARACUS lite can be upgraded with autosampler;
2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 - 50 μL), microliter pickup (1 - 35 μL)
|column||stainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD|
|column oven||20 - 100 °C, accuracy 0.1 %|
|reactor temperature||20 - 140 °C|
|spectroscopic detection||measuring range: high, low LED photometer (570 nm or 500 nm), or dual photometer (440 # 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty|
|detection limit||< 40 pmol / 20 µL with Ninhydrin|
|quantification limit||> 100 pmol / 20 µL with Ninhydrin|
|pump system||single pump reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 - 10.00 mL/min|
|maximum operating pressure||120 bar|
|ready to use kits||kits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 - 1000 runs|
|columns & eluents||Compatible with all High-Speed and High-Resolution columns, operating with all eluents based on different ionic strength (Li, Na, K)|
|control software & data aquisition||Clarity (21 CFR part 11 compliant) or AminoPeak|
|dimensions, weight, power||1080 x 370 x 375 mm*, 60 kg, 110 - 230 V|
|*800 mm with opened main unit|
Chromatographic separation of amino acids is performed by ion-exchange chromatography, followed by post-column derivatization with ninhydrin (Stein & Moore) and photometric determination using LED.
Yes, this is possible as with all ARACUS systems running on manual injection.
The autsoampler includes 2x 48 sample vials, which have a capacity of 1.5 mL. For small sample volumes, inlets can be used for the sample vials to ensure proper sample collection.
All applications dealing with amino acid analysis can be covered. These include the analysis of food and feed, the analysis of clinical samples such as blood plasma, urine or spinal fluid as well as research questions.
The ARACUS lite uses only a single photometer with a wavelength of 500 nm instead of two photometers with wavelengths of 570 and 440 nm. As an option, we offer a dual photometer that covers both wavelengths. In addition, the system has smaller bottles for the eluents, which eliminates the eluent rack and makes the system even more compact.
The system is requested when there is a lower volume of samples.
If the system is operated with the Clarity software, this option is possible. Contact us.
The system has an alarm system with three different levels. At "yellow" the user is informed about a situation that is not critical, at "orange" a controlled shutdown of the system is performed and at "red" the system is secured against serious damage by emergency shutdown.
The system works with a pre-sequence, the analysis sequence and a post-sequence. When the measurement sequence is completed, the system goes into standby and runs a circulation to keep the system in an equilibrated state to be able to start a new measurement again as quickly as possible.
You can determine the heating profile of the column oven, the temperature of the reactor, the flow of the eluents, and the selection of the eluents yourself. Optimized programs are delivered with the system as standard.