Amino acids for myocardial infarction and diabetes type 2

Sample Preparation and Analysis

500 µl blood plasma and 100 µl 4% dithiothreitol solution were mixed in a 1.5 mL tube and incubated by 40° for 30 min. Bound forms of homocysteine in the sample are reduced in form of free homocysteine by the use of 4% dithiothreitol solution. After the incubation 150 µL of precipitation solution were added and deposit in the refrigerator for 20 min for the protein precipitation. Then 500 µL of sample dilution buffer (including internal standard norleucin, 100 nmol/mL) were added and mixed. The supernatant was filtered with a membraSpin by centrifugation at 14000 rpm for five minutes. The particle free solution was used for the injection.

The samples were analyzed by the Amino Acid Analyzer ARACUS, manufactured and distributed by membraPure GmbH worldwide. ARACUS is using the classic routine analysis of amino acids by post-column derivatization with ninhydrin and the detection at 440 nm and 570 nm.
The use of post-column derivatization with ninhydrin is a well-established method to accurately quantify amino acids, including homocysteine, in clinical samples (Cohen & Michaud, 1993).

Aracus

Amino acid concentration of valine, homocysteine, methionine, allo-isoleucine, isoleucine, leucine, tyrosine, and phenylalanine in nmol/mL plasma of patients without myocardial infarction.

Amino acid concentration of valine, homocysteine, methionine, allo-isoleucine, isoleucine, leucine, tyrosine, and phenylalanine in nmol/mL plasma of patients with myocardial infarction.

Table 3: Amino acid concentration of valine, homocysteine, methionine, allo-isoleucine, isoleucine, leucine, tyrosine, and phenylalanine in nmol/mL plasma of patients without Diabetes Type 2.

Table 3: Amino acid concentration of valine, homocysteine, methionine, allo-isoleucine, isoleucine, leucine, tyrosine, and phenylalanine in nmol/mL plasma of patients without Diabetes Type 2.

Table 4: Amino acid concentration of valine, homocysteine, methionine, allo-isoleucine, isoleucine, leucine, tyrosine, and phenylalanine in nmol/mL plasma of patients with Diabetes Type 2.

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